Chevron Left
返回到 Basics of Extracellular Vesicles

学生对 加州大学尔湾分校 提供的 Basics of Extracellular Vesicles 的评价和反馈

328 个评分


This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....



Jan 10, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.


May 16, 2020

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works


1 - Basics of Extracellular Vesicles 的 25 个评论(共 89 个)

创建者 Shukhman B

Nov 12, 2016

创建者 Aedrian A

Jan 22, 2021

创建者 Margo M

Jun 29, 2021

创建者 Denise S

Mar 16, 2017

创建者 Minos V

Jan 31, 2018

创建者 Madhura K

Aug 5, 2020

创建者 Florencia I

Jun 30, 2019

创建者 Sandra M D A

Mar 8, 2019

创建者 Morgane G

Aug 16, 2020

创建者 Cahyani G A

Jun 5, 2022

创建者 Manasa V

Jun 29, 2021

创建者 Judith D M

Jan 1, 2018

创建者 Sai D K

Apr 15, 2022

创建者 Priyanka B

Apr 10, 2020

创建者 Samantha S

Aug 29, 2016

创建者 Momchil N

Mar 28, 2020

创建者 Santharam K S

Jun 9, 2020

创建者 Hasanul B

Oct 14, 2016


Nov 8, 2021

创建者 Esteban O

May 15, 2019

创建者 Azalea K

Jul 27, 2018

创建者 Nima K T

Feb 20, 2021

创建者 Jai M

Jan 2, 2017

创建者 Ashley R

Jun 30, 2017

创建者 Thais S M L

May 26, 2020